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The low regeneration potential of the central nervous system (CNS) represents a challenge for the development of new therapeutic strategies. Mesenchymal stem cells (MSCs) have been proposed as a possible therapeutic tool for CNS disorders. In addition to their differentiation potential, it is well accepted nowadays that their beneficial actions can also be mediated by their secretome. Indeed, it was already demonstrated, both in vitro and in vivo, that MSCs are able to secrete a broad range of neuroregulatory factors that promote an increase in neurogenesis, inhibition of apoptosis and glial scar formation, immunomodulation, angiogenesis, neuronal and glial cell survival, as well as relevant neuroprotective actions on different pathophysiological contexts. Considering their protective action in lesioned sites, MSCs' secretome might als...

The low regeneration potential of the central nervous system (CNS) represents a challenge for the development of new therapeutic strategies. Mesenchymal stem cells (MSCs) have been proposed as a possible therapeutic tool for CNS disorders. In addition to their differentiation potential, it is well accepted nowadays that their beneficial actions can also be mediated by their secretome. Indeed, it was already demonstrated, both in vitro and in vivo, that MSCs are able to secrete a broad range of neuroregulatory factors that promote an increase in neurogenesis, inhibition of apoptosis and glial scar formation, immunomodulation, angiogenesis, neuronal and glial cell survival, as well as relevant neuroprotective actions on different pathophysiological contexts. Considering their protective action in lesioned sites, MSCs' secretome might als...

Although it is hypothesized that mesenchymal stem cells’ secretome plays a major role in CNS regeneration, little is known on the mechanisms that regulate these actions. In the present work we aimed to assess if the secretome of a population of MSCs isolated from the WJ of the UC (HUCPVCs) was able to modulate neural/glial survival differentiation and proliferation in in vitro and in vivo models. Two approaches were used: (1) primary cultures of neurons and glial cells, were incubated with conditioned media (CM) from HUCPVCs and (2) HUCPVCs or their CM were injected in the dentate gyrus of male Wistar rats. In vitro results revealed that HUCPVCs CM increased cell proliferation in neuronal and glial cells cultures, as well as the survival of astrocytes, oligodendrocytes and neurons. In vivo experiments revealed that animals injected wit...